Saturday, October 5, 2019
Case Study - Mental Dysfunction Example | Topics and Well Written Essays - 2500 words
- Mental Dysfunction - Case Study Example This incident also changed him from being a happy boy into an angry fellow. In addition, it has made him to experience sleepless nights and loose the hope of looking a better future as indicated by his inaction in seeking for a job since that the incident. The sexual harassment incident has also made Isaac to be paranoid, aggressive and agitated. Freeman and Garety (2004) state that paranoia is characterized by excessive suspicion of the actions of other people around a person and hostility towards them and these can be noted in Isaac. He is distrustful and feels very insecure for example, he feels his mother has evil intentions towards him in her actions and accuses her of vague conspiracies. He thinks people in town see him as a gay. Evidence that Isaac is suffering from aggression can be seen in his actions like threatened his mother with a knife, causing problems in the streets and shouting around. He has also turned into behaviours like heavy smoking of marijuana to make him for get about all his bad lucks. These characters contrast his pre-harassment behaviours of being well mannered and trouble free. Isaacââ¬â¢s case presents the need for a good safety plan for him, the nurse in attendance and other local emergency department staff. It is evident that Isaac is suffering from depression. ... It will eliminate the possibilities of him chasing around the staff. A restoration of the confidence to interact with friends is important because it will make Isaac to cooperate willingly with the departmental staff instead of seeing them as enemies and incite him to fight them. Goldberg (1998) advices that Isaac should be handled with love to reduce his symptoms of aggression which are a sign of inner bitterness and anger. It will also stop him from becoming suspicious of the intentions of the actions of staff towards him hence he will not react negatively. However, Isaacââ¬â¢s hands should remain tied until he shows signs of improvement and a good level of relaxation. As soon as he becomes calm, Isaac should be given restricted freedom within the facility. This should be accompanied by activities that encourage restoration of self-efficacy like personal hygiene. In order to restore a good interpersonal relationship at this stage, he will be allowed to interact with his friends, neighbours, and mother under close supervision to gauge his level of risk once released to go home. The time periods will then be increased for every visit. He should also undergo behaviour therapy to reduce his paranoia state. Isaacââ¬â¢s Mental State Examination Appearance (dress, hygiene, grooming, posture, gait, eye contact) On his admission, Isaac was poorly dressed and very dirty. His hair was unkempt and had lice. Isaac was not able to maintain a stable posture and a straight eye contact for long. He paces back and forth too frequently and quickly turns to look away from the people around him. He is too slim for his age. Behaviour (manner, friendliness,
Friday, October 4, 2019
How Nursing Informatics has Affected the Telehealth Industry in the Research Paper
How Nursing Informatics has Affected the Telehealth Industry in the Past 5 Years - Research Paper Example Telehealth technologies also have both clinical and nonclinical uses. Few clinical uses for telehealth technologies include transmitting images for assessment and diagnosis, transmitting clinical data for assessment, diagnosis, or disease management, providing disease prevention and promotion of good health, using telephonic health advice in emergent cases, and using real time video (McGonigle & Mastrian, 2009, p. 290). These can be applied in numerous cases, from health education down to consultation and management. Clinical application of such technologies is seen to greatly benefit the client and the care providers as well. Telehealth technologies are also used in nonclinical settings. For instance, distance learning through such technology is now offered by several academic institutions around the globe. Conferences among telehealth workers can also be done, as well as information gathering and data management. Telenursing: Nursing informatics in Telehealth When nurses use telehe alth technology in providing care to patients, the term ââ¬Å"telenursingâ⬠comes into place, which is defined by the International Council of Nurses (ICN) as ââ¬Å"the use of telecommunications technology in nursing to enhance patient care; involves the use of electromagnetic channels to transmit voice, data, and video communication signalsâ⬠(as cited in American Telemedicine Associtation [ATA], n.d.). This modernization has changed the delivery of nursing care and interventions to certain clients. The presence of telenursing has given impact to several areas in practice of the field, and seeing its benefits, nursing organizations have expressed their encouragement in the use of such... This essay approves that the presence of telenursing has given impact to several areas in practice of the field, and seeing its benefits, nursing organizations have expressed their encouragement in the use of such technologies. Both informatics nurses and telenurses are now seen as rising nursing opportunities that could improve the practice. Several hospitals nowadays have telemonitoring equipments, bedside terminals, and automated clinical records being used by nurses, with systems varying depending on the complexity of the unit, needs of the patients, and the resources of the hospital. This report makes a conclusion that technology has innovated so many systems in the modern world, that it has invaded even the health care system. The telehealth industry is used in both clinical and nonclinical areas through several ways that would foremost benefit the clients and the health providers as well. Nursing informatics used in the delivery of care has been further enhanced by telehealth, in what is now known as telenursing. Telenursing allows nurses to care for more clients in a lesser amount of time despite distance through telemonitoring and tele-home care in communities. Formal education is also offered by some schools nowadays in order to enhance nursing competency in IT-based patient care. Indeed, the merge of nursing informatics and the telehealth industry changes nursing care delivery to clients, and will continue to change as the field advances.
Thursday, October 3, 2019
Conceptualization of the study Essay Example for Free
Conceptualization of the study Essay Traditionally since its inception, The Black Church in America has been a symbol of unity in the black community. In times of crisis it has cut across various religious positions and has served as a spiritual base camp for blacks in America for many years. It has also played a role in community participation, social activities and provided care for the sick and a place of refuge for the poor. During the civil rights movement the black community looked to the church leaders as an instrument that could effect change for their social conditions. During that time, black church leaders were influential to many of the social changes that we partake of today. Any important institution of society will have a number of roles and functions, and thus, an impact upon its membership. The Black church is no exception. In his study of the Black church, distinguished social scientist E. Franklin Frazier included a chapter, ââ¬Å"The Black Church: Nation within a Nation,â⬠in which he saw the church as an ââ¬Å"agency of social control,ââ¬â¢Ã¢â¬â¢ as an ââ¬Å"economic cooperative,ââ¬â¢Ã¢â¬â¢ as an ââ¬Å"educational institution,ââ¬â¢Ã¢â¬â¢ as an ââ¬Å"arena of political life,ââ¬â¢Ã¢â¬â¢ and as a ââ¬Å"refuge in a hostile white world: (Frazier, 1964). These functionsââ¬âpolitical, economic, social, and securityââ¬âall attest to the secular scope of the Black church. Some students of the Black church have supported this view of the churchââ¬â¢s secular function. An example is Joseph Washington, Jr. (1964), referring to the work of the late Rev. Martin Luther King, Jr. as an extension of the religious role of the church into the civil rights area. Although Washington viewed Kingââ¬â¢s philosophy as a perversion of the uses of Christian theology, and tended to view this activity as negative, his rationale was based on a lack of emphasis on theology in the Black church. Other writers have tended to support the view that, the function of the Black church was not so much to foster the spiritual growth of its members by its adherence to and development of the normal Christian theology of the church, as it was to serve their spiritually-related secular needs. St. Clair Drake and Horace Cayton, for instance, say in their study of the Black church in Chicago in the 1930ââ¬â¢s: The Negro Church is ostensibly a ââ¬Å"religious organization,ââ¬â¢Ã¢â¬â¢ but Bronzeville expects it, too, to ââ¬Å"advance the race. â⬠Negro Baptists think of their congregation as a ââ¬Å"Race Church,â⬠and their leaders concern themselves with such matters as fighting the job ceiling and demanding equal economic opportunity as well as ââ¬Å"serving the Lord. â⬠(Drake and Cayton, 1970 Pg 167). Kelly Miller also says that the Black church ââ¬Å" was not yet able to formulate a theological statement of its doctrine,ââ¬â¢Ã¢â¬â¢ that it broke from the white church and ââ¬Å"decided to go worship God under its own fig tree,â⬠and in so doing, developed a distinctive orientation (Miller, 1968). Others, however, such as Gayraud S. Wilmore and James Cone, built on the Drake and Cayton notion of the Black church as a ââ¬Å"race church. â⬠They outlined the tendency of the Black church to be responsive to the dominant forces in the Black community by pointing to the compatibility between the ââ¬Å"Blacknessâ⬠of the church and the dominant ideologiesââ¬âsuch as Black powerââ¬âin the community (Wilmore, 1973; Cone, 1969). Assuming these notions are true, it should be possible to test a theory of the responsiveness of the Black Church. Learning more about the function of the Black church is made urgent by the increased necessity for it to be responsive to the social needs of the Black community. The nature of these social needs is made urgent by the many new problems, of this age. STATEMENT OF THE PROBLEM AND PURPOSE Todayââ¬â¢s Problems which relate to the role of the church in secular activities is in its relationship to black families. The number of black families headed by black females has continued to rise over the years verses other ethnic groups. This dramatic rise may be attributed to an increase in babies born out of wedlock, divorce and separations, all of which have more serious underlying causes, and which also have ramifications on the ability to maintain adequate income for provisional needs. The church formerly was the home for many entire extended families in the Black community; as such, it played a role in establishing standards of conduct; legitimizing births, marriages, and deaths; educating and caring for children; and counseling married individuals. Consequently, the church was a resource center for the Black family, and played a role in its survival. If there are now new pressures on the family whichââ¬âin addition to employment-related difficultiesââ¬âthreaten the survival of the family, it is also worthwhile to assess the modern role of the church and the extent to which it provides services to meet these new needs. In light of the current problems facing the Black community, it is appropriate and necessary that a new assessment be made of the various institutions within the community which played a role in its progress. PURPOSE OF THIS STUDY The purpose of this study is to ascertain the present-day role of the Black church as a community institution, particularly with respect to the views of the churchââ¬â¢s leadership, membership, and individuals in the community. To what extent, if any, does the church leadership believe that the Black church should be involved in the community? How do church members perceive the scope of the church as it relates to non-secular activities? And to what extent do residents, especially non-church members, view the church as a community resource? IMPORTANCE OF THE STUDY 1. To gain insight of how church leaders and members, perceive the role of the Black church in the 21st Century. 2. To understand the communities view of what role the church should play in the political, social and economic activities of the community. 3. To define to what extent the black church will sponsor religious and secular programs. ASSUMPTIONS This study was prepared based upon the following assumptions: 1. To assist the church in bridging the gap and being more effective in its mission to serve through its activities. 2. To broaden the awareness of church leaders and members on the needs of the black community it serves. 3. To demonstrate to local leaders the contributions the black church has to offer for the welfare of its citizens. 4. To identify church programs that shows potential for future community growth. LIMITATIONS OF THE STUDY The study will be limited in the following way: 1. The primary focus of this study is to identify the impact of the black church in the community in which it exists. DEFINITION OF TERMS The following terms are defined to clarify their meaning in the context of study: Culture: The acquired knowledge, traits and symbols of a society Community: A group of people living in the same locally and under the same government. Secular: Not specifically relating to religion or to a religious body. METHOD OF STUDY This action research study will utilize action research, implementing quantitative research techniques. Quantitative research was selected for this study because it generates the comprehensive data needed for verification of results. ACTION RESEARCH The primary research method selected for this study is action research, because action research is concerned with improving a specific situation. Isaac and Michael validated the appropriateness of action research by confirming that it provides an organized structure to develop new skills or new approaches and so to solve problems with direct application to the classroom or working world setting. They suggested an orderly framework for problem solving and new developments that are superior to the impressionistic, fragmentary approach that typifies developments in education (1974, 13-30). Lewin, however, is credited with developing the term action research in 1944. He described the method as a cyclic operation in which the researcher is a participant in the process rather than a disinterested observer. The following steps are included in an action research study: (1) a planning or conceptualization step; (2) research or a fact-finding step; (3) an evaluation step; and (4) an execution step (1948, 34-36) Fermin and Levin in 1975 further described the research design as a preliminary and flexible strategy of investigation strategy of investigation or plan of action. The research is aimed at discovering ââ¬Å"why,â⬠and as a result, focuses centrally upon explaining social or political phenomena with answers of a theoretical nature. The research operates at a fuller or higher level of description, which includes making rational statements containing reasons or causes for behavior. Borg argued that action research design has as the chief benefit (1) a practical solution to a problem that may lead to a permanent improvement in the research environment; (2) it is the most appropriate method when practical significance is required rather than statistical and (3) the applicability of the finds are immediate and often of permanent improvement (1963, 319-322). QUANTITATIVE RESEARCH Isaac and Michael described quantitative research as allowing the researcher to formulate research questions or hypotheses and then to test each under controlled conditions. They decided its greatest merit is the convincing nature of hard data collected that can speak for itself (1974, 354-355). PROCEDURES To conduct this study, the following actions will be taken regarding each of the objectives: First objective: To identify and report the desired behaviors required for future Christian education leaders. 1. The literature of the field will be reviewed in the following areas: (a) Biblical context, (b) Church History, and (c) contemporary ministry. 2. The findings of the literature will be reported in chapter 2. Second objective: To validate the behaviors identified in the literature review. In order for the inventory of behaviors to be comprehensive, Flanagan stated that it is necessary to obtain a sufficiently representative sample (1949, 343). a) The degree of mutuality existing among the behaviors reported will be identified with respect to the different clusters. b) The number of behaviors will continue to be reduced by combining similar behaviors and writing new descriptions without regard for specific categories. c) The final inventory of administrative behaviors will be produced by the researcher. 2. The findings of the inventory of behaviors will be reported in chapter 3. Third objective: To make appropriate recommendations on the selection and training of future education administrators. 1. The study, including the findings of objectives 1 and 2 will be reviewed and summarized. 2. Appropriate conclusions will be drawn. 3. Specific recommendation which emerge from the study will be made, including suggestions for utilization of the competency areas recommended by the study, and suggestions for further research. 4. The summary, conclusions, and recommendation including check list will be reported in chapter 4. ORGANIZATION OF THE STUDY The study will be organized into four chapters in accordance with the Action Research, as table 1 displays. TABLE 1 Organization of the study based on action research Action Research Steps Chapters 1 2 3 4 Planning/Conceptualization X Fact Finding X X X Evaluation X Chapter 1 has stated the problem and purpose, explained the importance of the study, and stated the method. Chapter 2 will survey the literature to identify and report behaviors required to effectively analyze the characteristics of the church leaders, church members and surrounding neighborhood. Chapter 3 will report the validated behaviors for the role of the church. Chapter 4 will review and summarized the study, offer appropriate conclusion and discuss recommendations for change and future study. CHAPTER 2 CHARACTERISTICS We expect every church planter either black or white to be a good leader. Everyone else who knows about leader and his ministry, even including his group people expect it too. This is truly right no matter who a church planter is, may be any man or woman, may be a minister having both in church and outside of the church jobs. He may be a permanent pastor. Every church planter must enquire about his personality by self-questioning in such a personal way so that obtaining answers can help him to be sure about his decision and can help him to find out his true calling these answer can also help planter to know his future as a leader of his group. Our own understanding taught us about good leaders and leadership which we learned by working with different leaders. These leaders are the ones that people have known, read or watched about them. A successful leader has four elements including organization second program third morale and forth leadership. The greatest of four is leadership. Although morale is condition of mind and character that shows the person is brave. It also includes ability to do things with full trust on your self. The organizational element is based on morale followers; program of a church goes where the leadership goes. People have studied different kinds of leaders in secular history and in the Bible. The Lord Jesus Christ is their example as a true leader in Christian service. In the Bible God chose many people as leaders in His work for example Abraham, Noah, Nehemiah, Joseph, Paul and Daniel. Whenever the God leads anyone to think, about going into the Christian ministry he must look for a person who could demonstrate Him, what His person really used to be. A question also rises of what does God require of that person as His leader? And what it takes to be a good leader? The needed qualities of a Christian leader or a church planter should be as the Joseph had he even became a picture or copy of Jesus Christ. Josephs acts and character were good qualities for a church leader any church leader with these characteristics will definitely have success. ââ¬Å"It is vital that counselors who collaborate with African American congregations understand that titles and the values assigned to them can be very important in the Black church and within many African American families. Counselors who do not use the formal titles of ministers, church leaders, older church members, and members who have earned doctoral degrees run the risk of hampered rapport. â⬠(2005, 147) The God selected some of His people to be prophets, apostles, pastors, missionaries, and teachers, so that His people would be taught to serve and would become strong. This will carry on until we are united on accepting the Son of God by our faith. All the church has realized the need for leadership; they spend a big part of their life with the life of the church. People often think about the job of church leaders without attempting to connect their roles to the nature and purpose of the church. It means that church leaders become functionaries rather than ministers and become organization maintainers rather than church mission leader. But true black church leader as a person is that who uses his gifts to facilitate others in ministry, and then making suggestions and observations for implications to church. TESTIMONY OF PERSONAL CONNECTION WITH GOD When we talk about a church leader having a testimony, it proofs that God is with the leader and leader is with God. A church leader seeks a leadership ministry in Gods service and discusses his personal experience in the Christ. Sharing of a Christian testimony with others in public is the result of that personal experience. ââ¬Å"The LORD was with Joseph, and he was a prosperous man; and he was in the house of his master the Egyptianâ⬠(Genesis 39:2) Private and public life of a church leader, as a Christian, supports his testimony and influences others to become Christian. People expect church leader to be person who follows words and prayers of God. A church leader demonstrates people by giving example of personal closeness to God in his private and public. People judge their weaknesses with the help of purposeful Christian testimony of the leader. A church leader witnesses for Christ through his hands, lips, works and entire life. STRONG CERTAINTY AS A CHURCH LEADER Certainty represents freedom and surety of fact from doubt. It means a church leader is ahead of all other people sent by God. A church leader has a sense of call to be Gods leader so it gives him a special desire for leadership ministry. It also gives him a deep faith that God has His hands in his progress. It helps in making him sure that God wants him to be a church leader. ââ¬Å"â⬠¦. God sent me ahead of youâ⬠¦.. to preserve for you a remnant on earth â⬠¦Ã¢â¬ ¦.. it was not you who sent me here, but Godâ⬠(Gen 45. 5,7,8). His firm belief gives him inner faith and courage and helps him to go through hard times in his work by staying firmly on the job until finish it according to Godââ¬â¢s order. He does his job with dignity and proud character and blessings of the God who asked him to lead and serve. ââ¬Å"Paul, a bond-servant of Christ Jesus, called as an apostle, set apart for the gospel of Godâ⬠(Romans 1). The ministry requires church leaders to be certain of their calling as Gods leaders. INTEGRITY BEING A PART OF PERSONALITY Integrity is being truthful and honest. It also refers moral excellence and living and doing in a right manner. A church leader is true to him-self and keeps him-self pure and honorable. He knows the importance to keep integrity in front of God and people. Integrity as a needed quality for church leaders, and a church leader is free from any blame. He stands for God before his group and the people inside and outside of the church. A church planter makes a new church healthy when he has integrity in his private and public life. He knows how important it is for him to have authority as a leader, not only for just leadership but a church leader shows it in daily life. People need church leaders having integrity in their personality to teach them the difference between the right and wrong and help them choosing the right. DISTINCT CAPABILITIES When we talk about capabilities it represents fitness or ability. Distinct capability of a church leader enables him to do his job in a good manner wherever he serves people. Church leaders are good mentors they are wise and trusted persons who give advices. A church leader keeps on improving his talent by using his mentor and measuring obtaining results. People think that the greatest ability of a church leader is that, he depends only on God to avoid making mistakes in delivering his services to both God and people. Ability for church leading or planting is one of the parts of learnt talents of a church leader. USE OF AUTHORITY A church leader should use his authority properly which is given by the God and community. A church leader is not a good leader if no one is following him. People obey him because of his owning authority and their trust on his authority. Church leaders know above all else, that their power as a leader came from God. They receive authority and become Godââ¬â¢s witnesses when the Holy Spirit appears on them. A church leader understands that his leadership power comes from the God and understands that those, whom he serves, give him authority as a leader. ââ¬Å"A Christian does not forget that his leadership depends on responsibility that goes with authorityâ⬠(2-corinthians-5:9) A church leader uses these blessings for establishing of the groups or churches, his position as a leader is to achieve success in carrying out Gods purpose. If a church leader uses his authority in a wrong or bad use then he will definitely suffer for his doings. The church leaders should not forget that their leaderships depend on responsibility and their authority goes with equal responsibility. It means they should be able to believe and precede these authorities in a reasonable manner. ACCOUNTABLE A church leader is responsible for using church assets and other resources in an appropriate manner ââ¬â he should be accountable for himself. So Pharaoh said to Joseph, Im putting you in charge of the whole land of Egyptââ¬â¢ (Genesis 41:41). They trusted Joseph because of his self accountability. People who follow and support a church leader do not question about his plans for them. He opens his books for others to see and believes that honesty is a good policy. Church leaders spend their life on good and respected principles, the God and people expect that every church leader must have all of the qualities of Abraham, Noah, etc. A Church leader in leadership position is believed accountable and honest Christian leader. He needs to love the Godââ¬â¢s messengerââ¬â¢s reports or reviews and do what they do. The reports or reviews help him to improve from good to a better leader. A church leader makes decisions according to religious possibilities; he accepts responsibility for the obtaining result of his decisions. People usually do not consider religious aspects in their routine they read religious books like open letters. A church leader knows how to make good decisions, he then lives with the decisions that he has made and accepts responsibility for his works. PLANS AND DIRECT ACTIONS A church leader knows how to plan and direct actions to avoid any mistakes being made. He formulates and deploys plans to achieve objectives ââ¬â he directs the work accordingly until it finishes. He creates different questions in his mind; for example, what to do, why to do it, when to do it, how to do it, who to do, and how to finish it. A church leaderââ¬â¢s plans are simple, people who work as group members and other people who follow him understand his plans clearly. He forms teams and give them duties so that they can work with authority and responsibility. He makes rules that people accept to follow. The God wants a church leader to share His vision with his team members and people. A good church leader knows how to put the God given plan into practice. A church leader is the person who influences his team members and leads team members to carry out the agreed objectives, by following the example of the Christ. People under him follow his way of life; a church leader gets people to act on church and mission goals, and he also ministers the need of people. A Church leader humbly realizes that he needs Gods help and needs it in developing good plans that will work for others. These working plans then successfully meet the desired goals. A church leader does not build plans for other people to merely honor them, but he does it for the good of his people with everlasting results. UNCHANGED LOYALTY A church leader demonstrates loyalty that never changes. A church leader is faithful to his promises and duties. He never finds fault in his job; people respect him as a man of God living among them. A church leader gives his life to Gods mission and purpose and spends his entire life doing it. Loyalty being a leadership quality, a church leader is always loyal to God and his people. A church leader needs loyalty to start and grow a church or group. A loyal church leader is the base of a healthy church to grow and a key element for a church to produce other churches. A church leader influences others in his ministry through his loyalty to Jesus Christ as the Savior and Lord. A loyal church leader produces loyal members he starts and finishes his job with his team very well. A successful Christian leader is always keen and willing to work with a community or groups. He has a strong desire to develop community services together. USE OF GODââ¬â¢S GIFTS God has gifted all people with different abilities and characteristics. Nowadays, this divine gift directs people to serve churches, as ushers, church school teachers, financial officers, directors of food pantries, youth leaders, and in so many other roles. It all starts with gifts, which can be utilized in a variety of ways. Gifts are given by the God to everyone. The God doesnââ¬â¢t limit the gifts to his people, so we shouldnââ¬â¢t limit leadership to others. The potential to lead by using gifts for enabling others in ministry does not requires intelligence, education, economic status, age, gender, or any other human distinction. ââ¬Å"Children and youth, as well as adults, all are gifted. Women and men, boys and girls have all been gifted which they can use in leadership of a church. It does not mean, however, that everyone should be an administrator of a churchâ⬠. Gifts are several and of different nature, the variety of these gifts is much greater than churches usually realize. There are many gifts beyond the gifts that are usually used in preaching, teaching, and committee work. If one is to use all God given gifts for his leadership within a church, he/she must discover that wide range of gifts, and welcome the spirit to employ them. It is not always an easy task for a human to identify gifts since gifts have strange way of hiding themselves, particularly from those people who hold them. Godââ¬â¢s given gifts are so much a part of our personality that people not likely to think of them as gifts. For example, if a person is a good organizer, he must recognize this specialty and take it as a gift given by God. As Godââ¬â¢s gifts are discovered by interacting with others. However, discovering gifts is not enough because many people use there gifts haphazardly, with no attention to their development. Godââ¬â¢s given giftââ¬â¢s effectiveness can be significantly enhanced through intentional development of them. For example, if a person with natural painting ability can choose not to practice or take lessons and keep his ability as hobby and keeps his gift undefined from being fully utilized for service. The church leaders provide opportunities to people for training and development of Gods given gifts. They are intentional about using these gifts of children and youth in future leadership. The church leaders encourage their members to affirm and identify one anotherââ¬â¢s gifts. People discover and experiments their gifts in an atmosphere of acceptance provided by the church leader. A church leader expands his understanding in order to be comprehensive and not neglect peopleââ¬â¢s gifts. CHURCH LEADER ENABLES OTHERS A church leader uses his gifts to enable others to discover, develops, and use their Godââ¬â¢s given gifts. A church leader truly enables other people helping them to become Godââ¬Ës intended persons. This is not a true leadership to just manipulate others to act in a certain manner. A church leader puts aside his or own ego needs and coach others in such a way that help them blossom. The key function of a church leader is to facilitate others by helping them in discovering, developing, and effectively utilizing their God given gifts. Church leaders have five essential components to enable people. These are helping them to gain knowledge, encourage personal growth, assist them in working with others, aid in skill development, and provide appropriate settings. Here the term ââ¬Å"Knowledgeâ⬠is about information of the Christian traditions, quality Bible study, the nature and mission of the church, and the expert knowledge related to particular areas of services. The personal growth includes people growth as they develop awareness of giftedness, self knowledge, and understanding of purpose, which is firmly stable in a relationship with Jesus Christ. A church leader is able to work well with others; it is an essential element of the leadership. Skills development includes effective communication, managing small group, organization, and having healthy relationships with others that is beneficial for his ministry. A church leader gives importance to people using their gifts in such circumstance that improve potential of their success. The role of a church leader is not always immediate or direct. If a person who provides nurture to young children is providing early enabling for the leadership of others. Therefore, a church leader needs to be seen in his every task in terms of how it enables people for ministry. The Church leadership as a service may be a new perception for some people, so the church needs to have deep study that explores up Jesusââ¬â¢ own servant leadership model. A church leader must provide opportunities and safe places for members to develop and use their God given gifts. Discovering and developing Godââ¬â¢s gifts for people is not an end in itself. The ministry for which people are gifted is on a large scale, taking its place within and outside a church, even extends throughout the Godââ¬â¢s world. The black church is the one place in our community where people come together and pool their resources to better minister to the church and the community (1993, 54). There are mutual ministries among church members as accepting, loving, available, supportive, and accountable community of faith. Out of the churchââ¬â¢s boundaries, church leaders respond to human need by teaching love and hope, empowering the powerless, uplifting the poor, restoring creation, confronting all that harms persons and creation, and reconciling persons to God and one another. People note different implications for churches because the growth of effective leaders is grounded in a churchs vision of its ministry, a declaration of that vision needs not only to be created, but also acted on. Churchs involvements within and outside of the church provides a map of leadership opportunities by providing a starting point for selecting and developing leaders. COMMUNITY BEHAVIOR African American spirituality has woven collectively the best of African Anglo cultures to creatively shape their own African American identity. While black peoples have not been socially, politically or economically free in the history, the practice of spirituality has facilitated the development of different forms of cultural and spiritual freedom by interpreting, transcending and embracing, the constraints of an oppressive culture. Furthermore, the development of their own beliefs, norms, forms, practices, and structures has given rise to a culture and life that is a distinctive form of human survival, existence, and, freedom most importantly. The black churches as the centers of freedom influenced the praxis of African-American spirituality. They have become safe places where blacks gathered to embrace their combined concerns as a community of faith. They have been the only institutions in the African American experience that has continued relative independence from the domesticating influence of white pressure and overlords. Some black churches consider may be the last place to see integration due to the need to maintain a power base not available to black people elsewhere. After reviewing some aspects of worship as the context for cultural and spiritual freedom, black preaching in and out sides of the black churches are one of the most powerful expressions of freedom for black people in America. The ability to produce a soul language that defines truth into systems of meaning gives power and purpose to black people. Subsequently, Black community successfully adopts and adapts the structures of the much larger culture for their own purpose this practice comes from a double consciousness or translating. African Americans translated the experience of one kind to that of another which leading them to freedom. Therefore, black people spirituality gave freedom to the spirit and mind that the external condition could not take away. The religious approach is due to a deep faith in the sacredness of life that reaffirmed a personal dignity in blacks which resulted in the practice of nonviolence and mercy. Black community determines themselves how they responded to oppression. Various forms expressing improvisation and innovation from black soul forces constituted black culture. These forms under the subtitles black music, literature, humor, folk tales, sports, art and dance; and their worship forms under the headings of prayer, shouting, healing, dancing, singing praising and instrumental music transformation, teaching and preaching. These forms express that what is true and valuable will be determined by black community itself and will subvert and defy attempts of the larger culture to discern.
Social mobility
Social mobility What is social mobility? Drawing on different perspectives, assess the impact of the education system on opportunities and life chances on the basis of social class. What implications does this have for the work of helping agencies? Social mobility simply describes how people move along the social ladder. For this to happen there must necessarily be some form of social class in place. Social class occurs everywhere, even in the poorest communities. Take a typical village in Ghana for example, you will find that probably the village palm wine tapper or renowned farmer may be at the top of the social ladder; by village standards, they are the rich guys; these in addition could also serve as money lenders or movers and shakers. In the middle of the social ladder in the said village you might find the middle class who constitutes probably the majority of villagers with average incomes making just about enough from their farming or other vocations to make ends meet. Of course, at the bottom of this ladder will be the lower classes that have no jobs or qualifications and are at the mercy of the rich palm wine tapper. Social mobility describes how the classes move from their level of class upwards or downwards. This movement could involve the acquisition of new skills or education in the bid to get better jobs and hence get more income. Sometimes movement is caused by for example winning the lottery, an inheritance from parents or relatives or any procedure either deliberate or accidental which moves a person from one class to another. Social mobility or intergenerational mobilityas economists prefer to call it measures the degree to which peoples social status changes between generations. It is seen by many as a measure of the equality of life opportunities, reflecting the extent to which parents influence the success of their children in later life or, on the flipside, the extent to which individuals can make it by virtue of their own talents, motivation and luck. (Blanden J et al 2005) The Sociology guide has described Social mobility as a vital part of social stratification and an inseparable part of social stratification system because the nature, form, range and degree of social mobility depending on the very nature of the stratification system. Stratification system means the process of placing individuals in different layers or strata. In a social mobility paper Stephen Aldridge describes social mobility as a movement or opportunities for movement between different social groups and the advantages that go with this in terms of income, security of employment, opportunities for advancement etc. (Aldridge, 2001) There are types of social mobility. Intra-generation is when there has been a change in a persons social position. A typical example will be clerical assistant who works his/her way up in an organisation. However, if a persons social position changes over a generation it is called inter-generation mobility. An example is Margaret Thatcher and many others. She became prime minister as a grocers daughter. There appears to be significant intergenerational mobility in the United States, although perhaps less than is sometimes believed. Origins significantly affect destinations. Specifically, adult sons and daughters are more likely to look like their parents in terms of occupation or income than one would predict on the basis of chance. Still, there is considerable mobility. Indeed, even when occupations or income categories are broadly defined, a majority of adult offspring occupy a different occupational or income category than their parents.(Daniel P et al 1997) Horizontal mobility is another type of social mobility where a person changes their job-related position but does not change social class. An example is where a clerical assistant moves from Wellingborough to London and becomes an administrative officer. Vertical mobility on the hand takes the stage where people change their job-related position and change their social class as well. An example of vertical mobility will be for example a street cleaner becoming a solicitor or an army officer becoming a cleaner. They have fundamentally changed their socio-economic position. There are types of vertical social mobility. If someone moves down the social ladder it becomes downward mobility. When they move up on the social ladder it becomes upward mobility. For example if an Army officer is promoted in rank it becomes an upward mobility. The magazine Business Week in 2007 wrote an article about how mobile phones in Africa are creating high standards of living and boosting upward mobility. Only a few years ago, places like Muruguru didnt even register in the plans of handset makers and service providers. What would a Kenyan farmer want with a mobile phone? Plenty, as it turns out. To the astonishment of the industry, people living on a few dollars a day have proven avid phone users, and in many parts of the world cellular airtime has become a de facto currency. The reason is simple: A mobile phone can dramatically improve living standards by saving wasted trips, providing information about crop prices, summoning medical help, and even serving as a conduit to banking services. (Business Week, 2007) Another recent example of downward mobility is in this article in the telegraph. Though she is married to a builder, the 27-year-old housewife has rickety wooden planks for walls and covers her roof with plastic sheeting to keep out the rain in Harares Hatcliffe suburb far from the neighbourhood where she used to live. Right now I dont have a housing lot, but we are paying money to local co-operatives (to save for a down payment) so we may get lots to build houses, Chama said. And she is far from alone. According to official estimates, around two million Zimbabweans in this country of 12.2 million need accommodation. (Reagan Mashavave, 2009)- Another type of social mobility is structural mobility which involves vertical mobility but its movement is brought about by a major disorder. It can also be brought on by changes in society that brings improvement to a large number of people. Typical examples will be industrialisation, expansion of education and computerisation. These changes have all brought improvement to people in the UK and around the world. People have through it acquired higher social status and found higher paid jobs than their parents. There is also individual mobility which involves people being hindered from taking opportunities because of where they were live, their colour, gender, religion, their educational background, job, wellbeing and many others. The impact of the education system on opportunities and life chances on the basis of social class is enormous and hasnt changed much since education began. It is still difficult for working class children to access grammar and good comprehensive schools as the middle class and upper class have populated areas where these schools are placed. Childrens social class is still the most significant factor in determining their exam success in state schools, the Governments head of teacher training acknowledges today. In an interview with The Independent, Graham Holley, the chief executive of the Training and Development Agency, said: The performance of a school and a child in it is highly linked to social class. If you turn the clock back on pupils in school today 15 years and predict their outcomes from where they were born, you can do it. (Garner, 2008) Working class families are tied to the low paid jobs and often live in areas where schools are failing. Jobs are hard to find in these areas and its inhabitants are usually heavily dependant on benefits. Their lives are occupied with how to manage everyday living and not on reading to their children and giving them music and language lessons. The poorest children still have little chance of becoming lawyers, doctors, senior civil servants and financiers, a report published by the Liberal Democrats today shows. The Social Mobility Commission, set up by the party, said billions of pounds spent on improving social mobility over the past decade has helped middle-class rather than working-class children. Last year only 35% of pupils eligible for free school meals obtained five or more A* to C GCSE grades, compared with 63% of pupils from wealthier backgrounds.(Shepherd J, 2009) Disadvantaged children have little chance of watching educational programmes on television nor do they have the chance of reading the broad sheets. They are simply not patronised in their households. Libraries are rarely used and mobile libraries are not highly participated in working class areas. Areas in London for instance have seen various housing developments but they are not in the reach of the low paid. Overcrowding has many implications for the already struggling families as there is often no where to do homework. After school clubs charge for their services leaving low paid families out in the cold. Therefore chances of disadvantaged children reaching high levels of achievement in school are slightly dim. Young people in manual social classes remain under-represented in higher education in Great Britain. Despite increasing from a participation rate of 11 per cent in 1991/92 to 19 per cent in 2001/02, participation remains well below that of the non-manual social classes. Participation rates for the non-manual social classes increased from 35 per cent to 50 per cent over the same period. (http://www.statistics.gov.uk) The consequences of the manual or low class remaining under-represented in higher education is unthinkable as children from these background will experience either downwardly mobile or not move on the social ladder at all as a result of them not entering higher education. Todays job market is very competitive and even those with good qualifications are finding it difficult to hold onto their jobs. It means that most all white collar jobs will be held by the middle class and upper class families. All the independent evidence shows overall standards to be rising. But the bad news is that when it comes to the link between educational achievement and social class, Britain is at the bottom of the league for industrialised countries. Today, three-quarters of young people born into the top social class get five or more good GCSEs, but the figure for those born at the bottom is less than one-third. We have one of the highest university entry rates in the developed world, but also one of the highest drop-out rates at 16.(Independent, 8 September 2003) Anthony Giddens writes in Sociology and Social Mobility that education is not necessarily a means to an end. Education would have to work with other factors to foster social mobility. Education shouldnt be seen as a panacea for all societys problems. It has a significant role to play, but we cant hold schools and Universities solely responsible for promoting social mobility. Its important not to think of the education system as if it works in a vacuum factors like changes in employment and the economy, and the social determinants of childrens educational attainment, are critical in determining patterns of mobility.(Giddens, 2007) However, education definitely has opportunities for people to progress along the social ladder by providing relevant new skills, information, courses and therefore creating opportunities in life for them. A few years back a hair dresser did not need know too much about what she/he did as a hair dresser but in today environment he/she would have to know all the science there is to cutting, dressing and managing the business. Technology and other factors have raised the standard of work so high that without continuous professional development opportunities are not stretch far. In this new labour market, the value of college degrees overall is greater than ever before. Between 1984 and 2000, employment in jobs requiring a college degree grew by 20 million in the US, accounting for two-thirds of total job growth. Over the same period, wages for college graduates increased. In contrast, high school graduates in America who did not continue with education saw their wages fall below middle class levels for the first time. As a consequence, the opportunities for Americans with terminal high school diplomas are less than a generation ago.(Social Mobility Foundation, 2008) Although a small percentage of poor families are accessing higher education there is evidence that the UK government for instance is working frantically to improve the chances of the less privileged through innovations like Every Child Matters. This innovation is to give every child the chance of accessing education and other services in the community to give them better outcomes in life. Hence the establishment of Surestart Centres which is a one stop service for early education, childcare, health and family support. This Government has invested heavily in policies designed to give all children the chance to succeed. There have already been significant improvements in educational achievement, and reductions in teenage pregnancy, re-offending and children living in low income households. Todays children and young people experience wider opportunities and benefit from rising prosperity, better health and education than those in previous generations. The implications on the work of helping agencies are many. Helping agencies like Childrens Centres pick up the brunt of any inequalities that lay in society. We bid or vie for large sums of money to run various courses and projects that underpin social mobility. In the bid of helping families read we have set up borrow a chattersack in our childrens centre. This is to encourage parents who might otherwise not access libraries or buy books for their children. The books and toys are expensive but we charge a very minimal fee for them. Often people bring to us various problems like divorce, debt and housing issues which we do our best to refer to other agencies for further assistance. We hold classes with Citizen Advice Bureau to advise people on budgets and other financial incapability. Many of our clients are lone parents or young families struggling on low incomes. They are often in debt and or have little financial knowledge. They often live on large council estates where aspirations are not that great. The recent recession has hit these areas hard and some people are experiencing downwardly mobility. This comes with various demands like counselling, retraining and financial loss. Equality of opportunity is a sine qua non for any modern society and, in Britain, is a principle supported by all mainstream political opinion. Despite this, household income remains the biggest single predictor of a childs future success, and a recent report by the Sutton Trust found that, all too frequently, young people from financially disadvantaged backgrounds end up in a cul de sac of opportunity. (Social Mobility Foundation, 2008) Below is an example of some the types of work some helping agencies do. They use government funds to bridge the gap between rich and poor by organising trips to parks, educational establishment to boost confidence and increase knowledge. Nearly 175,000 bright children on free school meals will be given a chance at the age of 11 to visit a university as part of a drive to lift the aspirations of working-class people and increase stalled social mobility in Britain. Young people in the top 20% of ability based on test results, and who are eligible for free school meals, are about half as likely to go to university as those who are not eligible for free meals. They will now be offered two chances to visit universities. (Wintour, 2007) We organise sporting activities that will encourage the less privileged to put their feet in door of expensive sporting activities. We work with other agencies to bring information and understanding to parents who in effect calve their childrens place on the social ladder. A new report by the British think-tank Demos has hit the headlines, with its claim that Parents are the principal architects of a fairer society. Based on research from the Millennium Cohort Study, the report argues that how children are parented has a more significant impact upon their future life chances than just about anything else, including poverty and the social class into which they are born. (Bristow J, 2009) Helping agencies are faced with many demands for services they can and cannot provide. At our centre for instance there is the demand for certain services like computer classes, some sort of back to work training, cooking classes but we havent got the facilities and the man power to run such courses. We are therefore forced to send clients to other childrens centres in that losing their business. If we do manage to run any of the courses that put pressure on our facilities then we have to limit the numbers which in turn causes us to run the courses several times to fit everyone in. There is also a steady demand for information on sensitive family matters like finance. Education seems to be one the important factors manipulating social mobility. In todays society, education is becoming increasingly important as it used to ascertain the jobs people will end up in. Education is also used to determine peoples social class position. The recent government for instance has introduced many initiatives. Free child care for two year olds was trialed for sometime and is going to be offered to children from disadvantaged backgrounds. Back to work incentive of à £500 and the investment into early years, FE schools and workplace training are all initiatives which research has suggested that has not boosted social mobility. In 1999 Tony Blair told the Labour Conference: If we are in politics for one thing, it is to make sure that all children are given the best chance in life. A decade on, the Government has had to admit that billions of pounds of investment in nurseries and schools and on training has failed to bridge the class divide, and that social mobility in Britain has stalled. (Bennett and Bahra 2007) As mentioned before there are many factors contributing to this fact. One such fact is the advantage that middle class families have over poorer families when it comes to education. References Web Sources Daniel P et al (1997) Intergenerational mobility in the United States [online] Available from: http://www.urban.org/publications/406796.html [Accessed 16/12/09] http://www.businessweek.com/magazine/content/07_39/b4051054.htm?campaign_id=rss_tech[ Accessed 27/12/09] http://www.dcsf.gov.uk/everychildmatters/about/background/background/ [Accessed 16/12/09] http://www.polity.co.uk/giddens5/news/sociology-and-social-mobility.asp#intro[Accessed [16/12/09] http://www.statistics.gov.uk/STATBASE/ssdataset.asp?vlnk=7308 [Accessed 27/12/09] Jennie Bristow (2009) There is more to human character than sharing toys [online] Available from: http://www.parentswithattitude.com/parentsforum/tag/social-mobility[19/12/09] Non Web Sources (2003), Class still counts in Britain. Independent, Monday Sept 8 Bennett and Bahra (2009), Social mobility: Labour tries to revive flagging crusade to help poor children. The Times, Wednesday Jan 14 Blanden J et al (2005), Social Mobility in Britain Low and falling, Centrepiece 2005 Mashavave R. (2009) Downwardly mobile in Zimbabwe. Telegraph, Tuesday Dec 15 Richard G (2008), Social class determines childs success. Independent, Thursday Sept 18 Shepherd J (2009), Social class still determines success. Guardian, Monday Social Mobility Foundation (2008) A national project for social mobility Stephen A (2001), Social Mobility , A discussion Paper , Performance and Innovation Unit The Sutton Trust (2008) : Social Mobility and Education. London Wintour P (2009), Social mobility drive focuses on schools. The Guardian, Wednesday Jan 14
Wednesday, October 2, 2019
Comparing Characters in the Coen Brothers Millerââ¬â¢s Crossing and Willia
Comparing Characters in the Coen Brothers' Millerââ¬â¢s Crossing and William Kennedy's Legs The movie Millerââ¬â¢s Crossing and the novel Legs by William Kennedy have two characters that have a special quality, which adds dramatically to their characterization. The main character of Millerââ¬â¢s Crossing, Tom Reagan, and the main character from Legs; Jack Diamond shares many similar traits and symbolic equivalence. In particular they had an item that they wore or carried, and this item had the ability to show what is going on inside the characterââ¬â¢s mind. Not only did the items have the ability to tell what was on their mind; it also has the ability to foreshadow. At times in the novel where their mind/conscience was tested, the use of their items determined the outcome. The body can not live without the mind, so it is important for the characters to remain close to these items that symbolize their mind or they will surly die. Tom Reagan had a dream in which his hat fell off his head and the wind blew it away. He did not run after the hat in the dream. However in reality he kept the hat close to him at all costs. The dream is the opening to the movie and is explained during a conversation with Verna. During the movie he is seen with the hat and without. The trend his hat wearing follows with when engaging in sex it is off, and actually gets its own scene of being thrown on to a chair or something of that nature. For the most part, it is also seen without his hat when somethin...
Tuesday, October 1, 2019
A Comparison of Two Paintings from the Renaissance Period Essay
A Comparison of Two Paintings from the Renaissance Period Introduction à à à à à This paper will compare the themes found in the paintings ââ¬Å"Madonna and Child with St. John the Baptist and an Angelâ⬠by Domenico di Bartolomeo Ubaldini (Puligo) and ââ¬Å"Madonna Enthronedâ⬠by Giotto. Both paintings deal with fables from the Christian faith but were executed during different periods in art. The Giotto painting was created around 1310 and the Puglio painting was executed between 1518 ââ¬â 1520. Here, these two paintings have similar themes both at the extreme beginnings and endings of the Italian Renaissance, and as such they serve to present an exceptional example of the developments in art that occurred within that time. This paper shall compare these two paintings through addressing a series of questions on the subject. Subject Matter or Iconography à à à à à ââ¬Å"Madonna Enthronedâ⬠is the earlier of the two works to be surveyed in this paper, and as such there is a great deal more popularity surrounding this work. The image is simple: The subject matter is religious and concerns a host of holy figures surrounding the Madonna with an infant figure of Christ on her lap. ââ¬Å"Madonna and Child with St. John the Baptist and Angelâ⬠is similar in that other holy figures are gathered to pay their respects to Christ but the scene is more open and less focused on just the two central figures. ââ¬Å"Madonna and Childâ⬠¦Ã¢â¬ in my opinion is therefore a more complex composition, where instead of having two recogn...
Department of biology Essay
INTRODUCTION: Every cells of living organism contains genetic materials known as deoxyribonucleic acid, DNA. It can be isolated from tissue sample of living things by separating it from other cellular component in a manner that still preserves its structures. The structure of DNA is double-stranded helices that made up from the monomer of nucleotides. Each of the nucleotides composed of three parts that are the phosphate group, deoxyribose sugar backbone and also nitrogenous bases (Adenine, Guanine, Thymine and Cytosine). This nitrogenous base are arranged in sequence and holds the information and coding for controlling the physical trait that we all have as well as the regulation of our body. DNA extraction is simply a process that results in separation of DNA from the cells or viruses that are hosting it. Through the meaning is simple but the process is not. When we go into the peculiar details of DNA extraction, we realize that itââ¬â¢s more of an initial stage in other intensive DNA testing processes. DNA test could be performed for any reason; however for any DNA testing to happen the first stage normally is the isolation and extraction of DNA molecules from the cells that they reside in. DNA extraction follows a series of step, stripping all proteins from the DNA and the extraction protocols have to make sure that the DNA thus obtained via isolation and extraction is of high or acceptable quality. DNA isolation is a process of purification of DNA from sample using a combination of physical and chemical methods. Currently it is a routine procedure in molecular biology or forensic analysis. Primary structure consists of linear sequence of nucleotides that are linked together by phospodiester bonds. It is the linear sequence of nucleotides that make up the primary structure of DNA. Specific techniques must be chosen for isolation of DNA from some samples such as samples from microorganisms with thick cellular wall, for example yeast. Biological DNA represents the information which directs the functions of a living thing. A. Yeast DNA Extraction Materials : 1 packet of dry yeast, Sodium chloride, Meat tenderizer, Ice cold 95% ethanol, Sunlight detergent, distilled water, blender, graduate cylinders ( 10mL, 100mL and 500mL ), Beaker ( 250mL, 100mL ), glass stirring rod and wooden sticks, 15mL test tube, test tube rack, 1 ,l pipette and blue tips. Method: 1. 1 packet of dry yeast was mixed with 40ml of 50à °C tap water. The yeast was stir to dissolve and the mixture was leave and covered for 20 minutes. 2. Salt/detergent solution was prepared by adding 40 ml detergent and 40g NaCl to 360ml distilled water. The solution was mixed till dissolved. 3. 5% meat tenderizer solutions were prepared by adding 5g of meat tenderizer to 80 ml of distilled water. The solution was top up to 100 ml with distilled water. *salt / detergent solution and meat tenderizer solution is prepared once for Part A, B and C. *alternatively, 5% meat tenderizer solution may be substitute with 100 ml of fresh papaya juice or pineapple juice. 4. 40 ml of yeast mixture and 40 ml of salt/detergent solutions was placed in a blender and was blended at high speed for 2 minutes. 5. The solution was pour into the beaker and 15ml of meat tenderizer solution was added. The solution was stir to mix. 6. The mixture was leave at room temperature for 5 minutes. 7. A cheese cloth was place over a filter funnel. The mixture was pour over the filter funnel and the clear supernatant was collected. 8. 3 ml of clear solutions was transfer into a 15ml tube. 9. The test tube was tilted to a 45 degree position. 3 ml of 95% ice cold ethanol was gently added to the side of the tube. 10. The test tube was leave undisturbed for 3-5 minutes. A layer will be formed in the tube. 11. DNA precipitate was formed at the interphase layer. A wooden stick was used to swirl the DNA out Result : Table A(i) : Yeast DNA Extraction B. Onion DNA Extraction Materials : Fresh onion, salt detergent solution, meat tenderizer solution, ice cold 95% ethanol, distilled water, blender, graduated cylinders (10 ml and 100 ml), glass stirring rod and wooden sticks, 15 ml test tube, test tube rack, 1 ml pipette and blue tips. Method : 1. The prepared salt/detergent solutions and meat tenderizer solution from part A was gathered. 2. 3 medium sized onions were cut into an inch cube and were placed in a blender. 3. 100 ml of salt/detergent solution was added in a blender. 4. The solution was blended at high speed for 2 minutes. 5. A cheese cloth was placed over filter funnel. The mixtures were poured over the filter funnel and the clear supernatant was collected. 6. The clear solution was transfer into a beaker and 30 ml of meat tenderizer solution was added. 7. The mixtures were leave at room temperature for 5 minutes. 8. 3 ml of clear solutions was transfer into a 15 ml tube. 9. The test tube was tilted to a 45 degree position. 3 ml of 95% ice cold ethanol was gently added to the side of the tube. 10. The test tube was leave undisturbed for 3-5 minutes. A layer will be formed in the tube. 11. DNA precipitate was formed at the interphase layer. A wooden stick was used to swirl the DNA out. Result : Table B(i) : Onion DNA Extraction C. Apple and Orange DNA extraction Materials : Fresh apple, fresh orange, salt detergent solution, meat tenderizer solution, ice cold 95% ethanol, distilled water, blender, graduated cylinder ( 10 ml and 100 ml ), glass stirring rod and wooden sticks, 15 ml test tube, test tube rack, 1 ml pipette and blue tips. Methods : 1. The prepared salt/detergent solutions and meat tenderizer solution from part A was gathered. 2. An apple / orange were cut into an inch cube and were placed in blender. 3. 100 ml salt/detergent solutions were added in a blender. 4. The solution was blended at high speed for 2 minutes. 5. A cheese cloth was placed over filter funnel. The mixtures were poured over the filter funnel and the clear supernatant was collected. 6. The clear solution was transfer into a beaker and 30 ml of meat tenderizer solution was added. 7. The mixtures were leave at room temperature for 5 minutes. 8. 3 ml of clear solutions was transfer into a 15 ml tube. 9. The test tube was tilted to a 45 degree position. 3 ml of 95% ice cold ethanol was gently added to the side of the tube. 10. The test tube was leave undisturbed for 3-5 minutes. A layer will be formed in the tube. 11. DNA precipitate was formed at the interphase layer. A wooden stick was used to swirl the DNA out. Result : Table C(i) : Orange DNA Extraction Table C (ii) : Apple DNA Extraction RESULT ALL OF EXPERIMENTS: Sample Extraction Amount Apple Success Large Onion Success Small Orange Success Small Yeast Success Small DISCUSSION: Based on our experiment discussion, we obtained that the difference amount of DNA extraction from yeast, onion, apple and orange is different. The amount of apple DNA extraction is larger than onion, orange and apple DNA extraction. Each step of the process will help in a certain way to extract DNA, until we are finally successful in the end. In the yeast, extraction, the ethanol will be able to separate the DNA and it will float between the less dense ethanol and the denser homogenizing mixture. In the onion DNA extraction, the chloroform and homogenizing medium will help break down the cell membranes and the ethanol like in the yeast DNA extraction. It will cause the DNA to separate and be suspended in the interface between the two solutions. It will be same to apple and orange DNA extraction. The precaution step on this experiments we tilt 45à º to add the ethanol. This is because it will form a layer on top of the sample because the ethanol is less than water. So, it does will be on the top of layer. We also used 50ï⠰ of hot water in yeast solution. Its will formed the best of result because hot water is the optimum temperature for yeast. We also obtained about perceptions step why only clear solution produced in these experiments. Its means the extraction was failed. CONCLUSION: REFERENCES: http://www.whatisdna.net/dna-extraction.html http://classic.sidwell.edu/us/science/vlb5/Labs/DNA_Extraction_Lab/dna_extraction_lab.html#predictions http://sciencehk.weebly.com/lab-reports.html ANSWER AND QUESTION: Answer all the questions. 1. Describe the functions of following: a. Applying blender to sample and salt/detergent solutions Strain the DNA mixture. b. Salt Salty water helps the DNA precipitate (solidify and appear) when alcohol is added. c. Detergent Detergents are used to break down cell walls and nuclear membranes to release the DNA. They work by chemically poking holes in the cell membranes or walls. Once holes are poked in the membranes, the membranes can be further distrupted mechanically, as with a blander. After that, itââ¬â¢s easier to get the contents of the cell out, including the DNA, d. Meat tenderizer solutions Meat tenderizer acts as an enzyme. The DNA in the nucleus of the cell is moulded, folded, and protected by proteins. The meat tenderizer cuts the proteins away from the DNA. e. 95% ice cold ethanol Having ice cold ethanol only increases the rate of precipitation of DNA and helps increase yield of DNA. It can either use room temp or ice cold ethanol for DNA precipitation. Think about precipitation of a super concentrated solution as you decrease the temperature. As the temperature decreases, the amount of precipitation increases. Overall temperature affects solubility. As temperature decreases the substance becomes more insoluble (in general. this does not apply to every molecule). So, ice cold EtOH allows for more DNA to interact together and allow for a more rapid and efficient precipitation of DNA. 2. Compare the reliability amount of DNA obtained from all the samples. The reliability amount of DNA obtained from apple is much larger compared to Reliability amount of DNA from onion, orange and apple. 3. Write out the principle involved in DNA extraction. ââ¬â Break open cells by mashing the fruit. ââ¬â Dissolve organelles and cell membranes with detergent. ââ¬â Separate DNA from proteins with salt ââ¬â Filter out the clumps with a coffee filter ââ¬â DNA precipitates in cold alcohol and is spooled out.
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